Large-volume en-bloc staining for electron microscopy-based connectomics.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26235643.
- Also identified by DOI 10.1038/ncomms8923 and PMC identifier 4532871.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Large-scale connectomics requires dense staining of neuronal tissue blocks for electron microscopy (EM). Here we report a large-volume dense en-bloc EM staining protocol that overcomes the staining gradients, which so far substantially limited the reconstructable volumes in three-dimensional (3D) EM. Our protocol provides densely reconstructable tissue blocks from mouse neocortex sized at least 1 mm in diameter. By relaxing the constraints on precise topographic sample targeting, it makes the correlated functional and structural analysis of neuronal circuits realistic.
Medical subject headings
- Connectome
- Imaging, Three-Dimensional
- Microscopy, Electron
- Neocortex
- Neurites
- Neuroglia
- Neurons
- Staining and Labeling