Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26237515.
- Also identified by DOI 10.1038/nbt.3301 and PMC identifier 4532950.
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Abstract
Synthetic regulatory circuits encoded in RNA rather than DNA could provide a means to control cell behavior while avoiding potentially harmful genomic integration in therapeutic applications. We create post-transcriptional circuits using RNA-binding proteins, which can be wired in a plug-and-play fashion to create networks of higher complexity. We show that the circuits function in mammalian cells when encoded in modified mRNA or self-replicating RNA.
Medical subject headings
- Gene Regulatory Networks
- RNA
- RNA-Binding Proteins
- Synthetic Biology