Informatics for RNA Sequencing: A Web Resource for Analysis on the Cloud.
Where this comes from
- Record sourced from PubMed, PMID 26248053.
- Also identified by DOI 10.1371/journal.pcbi.1004393 and PMC identifier 4527835.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Massively parallel RNA sequencing (RNA-seq) has rapidly become the assay of choice for interrogating RNA transcript abundance and diversity. This article provides a detailed introduction to fundamental RNA-seq molecular biology and informatics concepts. We make available open-access RNA-seq tutorials that cover cloud computing, tool installation, relevant file formats, reference genomes, transcriptome annotations, quality-control strategies, expression, differential expression, and alternative splicing analysis methods. These tutorials and additional training resources are accompanied by complete analysis pipelines and test datasets made available without encumbrance at www.rnaseq.wiki.
Medical subject headings
- Computational Biology
- Internet
- RNA
- Sequence Analysis, RNA
- Software