Continuous directed evolution of DNA-binding proteins to improve TALEN specificity.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26258293.
- Also identified by DOI 10.1038/nmeth.3515 and PMC identifier 4589463.
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Abstract
Nucleases containing programmable DNA-binding domains can alter the genomes of model organisms and have the potential to become human therapeutics. Here we present DNA-binding phage-assisted continuous evolution (DB-PACE) as a general approach for the laboratory evolution of DNA-binding activity and specificity. We used this system to generate transcription activator-like effectors nucleases (TALENs) with broadly improved DNA cleavage specificity, establishing DB-PACE as a versatile approach for improving the accuracy of genome-editing agents.
Medical subject headings
- DNA-Binding Proteins
- Deoxyribonucleases
- Directed Molecular Evolution