Unbiased phosphoproteomic method identifies the initial effects of a methacrylic acid copolymer on macrophages.
basic_science · Level V
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- Record sourced from PubMed, PMID 26261332.
- Also identified by DOI 10.1073/pnas.1508826112 and PMC identifier 4553830.
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Abstract
An unbiased phosphoproteomic method was used to identify biomaterial-associated changes in the phosphorylation patterns of macrophage-like cells. The phosphorylation differences between differentiated THP1 (dTHP1) cells treated for 10, 20, or 30 min with a vascular regenerative methacrylic acid (MAA) copolymer or a control methyl methacrylate (MM) copolymer were determined by MS. There were 1,470 peptides (corresponding to 729 proteins) that were differentially phosphorylated in dTHP1 cells treated with the two materials with a greater cellular response to MAA treatment. In addition to identifying pathways (such as integrin signaling and cytoskeletal arrangement) that are well known to change with cell-material interaction, previously unidentified pathways, such as apoptosis and mRNA splicing, were also discovered.
Medical subject headings
- Biocompatible Materials
- Gene Expression Profiling
- Macrophages
- Phosphoproteins
- Polymethacrylic Acids
- Protein Processing, Post-Translational
- Proteomics