A Fluorescent Probe to Measure DNA Damage and Repair.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26309022.
- Also identified by DOI 10.1371/journal.pone.0131330 and PMC identifier 4550365.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
DNA damage and repair is a fundamental process that plays an important role in cancer treatment. Base excision repair (BER) is a major repair pathway that often leads to drug resistance in DNA-targeted cancer chemotherapy. In order to measure BER, we have developed a near infrared (NIR) fluorescent probe. This probe binds to a key intermediate, termed apurinic/apyrimidinic (AP) site, in the BER pathway where DNA damage and repair occurs. We have developed an assay to show the efficacy of the probe binding to AP sites and have shown that it can distinguish AP sites in DNA extract from chemotherapy treated cells. This probe has potential application in monitoring patient response to chemotherapy and evaluating new drugs in development.
Medical subject headings
- DNA Damage
- DNA Repair
- Fluorescent Dyes