Inntags: small self-structured epitopes for innocuous protein tagging.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26322837.
- Also identified by DOI 10.1038/nmeth.3556.
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Abstract
Protein tagging is widely used in approaches ranging from affinity purification to fluorescence-based detection in live cells. However, an intrinsic limitation of tagging is that the native function of the protein may be compromised or even abolished by the presence of the tag. Here we describe and characterize a set of small, innocuous protein tags (inntags) that we anticipate will find application in a variety of biological techniques.
Medical subject headings
- Epitopes
- Fluorescent Antibody Technique
- Immunoprecipitation
- Proteins