Grafted biomembranes containing membrane proteins--the case of the leucine transporter.

Jagalski, Vivien; Barker, Robert D; Thygesen, Mikkel B; Gotfryd, Kamil; Krüger, Mie B; Shi, Lei; Maric, Selma; Bovet, Nicolas et al. · Soft Matter · 2015

basic_science · Level V

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Abstract

Here, we bind the sodium dependent amino acid transporter on nitrilotriacetic acid/polyethylene glycol functionalized gold sensors in detergents and perform a detergent-lipid exchange with phosphatidylcholine. We characterize the LeuT structure in the adsorbed film by magnetic contrast neutron reflection using the predicted model from molecular dynamic simulations.

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