Promoter-Dependent Translation Controlled by p54nrb and hnRNPM during Myoblast Differentiation.
Level V
Where this comes from
- Record sourced from PubMed, PMID 26332123.
- Also identified by DOI 10.1371/journal.pone.0136466 and PMC identifier 4558007.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Fibroblast growth factor 1 (FGF1) is induced during myoblast differentiation at both transcriptional and translational levels. Here, we identify hnRNPM and p54nrb/NONO present in protein complexes bound to the FGF1 promoter and to the mRNA internal ribosome entry site (IRES). Knockdown or overexpression of these proteins indicate that they cooperate in activating IRES-dependent translation during myoblast differentiation, in a promoter-dependent manner. Importantly, mRNA transfection and promoter deletion experiments clearly demonstrate the impact of the FGF1 promoter on the activation of IRES-dependent translation via p54nrb and hnRNPM. Accordingly, knockdown of either p54 or hnRNPM also blocks endogenous FGF1 induction and myotube formation, demonstrating the physiological relevance of this mechanism and the role of these two proteins in myogenesis. Our study demonstrates the cooperative function of hnRNPM and p54nrb as regulators of IRES-dependent translation and indicates the involvement of a promoter-dependent mechanism.
Medical subject headings
- Fibroblast Growth Factor 1
- Heterogeneous-Nuclear Ribonucleoprotein Group M
- Internal Ribosome Entry Sites
- Myoblasts
- Nuclear Matrix-Associated Proteins
- Octamer Transcription Factors
- Promoter Regions, Genetic
- Protein Biosynthesis
- RNA-Binding Proteins