McCLEC, a robust and stable enzymatic based microreactor platform.

Conejero-Muriel, Mayte; Rodríguez-Ruiz, Isaac; Martínez-Rodríguez, Sergio; Llobera, Andreu; Gavira, José A · Lab Chip · 2015

basic_science · Level V

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Abstract

A microfluidic chip for cross-linked enzyme crystals (McCLEC) is presented and demonstrated to be a stable, reusable and robust biocatalyst-based device with very promising biotechnological applications. The cost-effective microfluidic platform allows in situ crystallization, cross-linking and enzymatic reaction assays on a single device. A large number of enzymatic reuses of the McCLEC platform were achieved and a comparative analysis is shown illustrating the efficiency of the process and its storage stability for more than one year.

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