Cas9 gRNA engineering for genome editing, activation and repression.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26344044.
- Also identified by DOI 10.1038/nmeth.3580 and PMC identifier 4666719.
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Abstract
We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.
Medical subject headings
- CRISPR-Cas Systems