Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale.
basic_science · Level V
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- Record sourced from PubMed, PMID 26344045.
- Also identified by DOI 10.1038/nmeth.3569 and PMC identifier 4626315.
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Abstract
Active DNA demethylation in mammals involves oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). However, genome-wide detection of 5fC at single-base resolution remains challenging. Here we present fC-CET, a bisulfite-free method for whole-genome analysis of 5fC based on selective chemical labeling of 5fC and subsequent C-to-T transition during PCR. Base-resolution 5fC maps showed limited overlap with 5hmC, with 5fC-marked regions more active than 5hmC-marked ones.
Medical subject headings
- Cytosine
- DNA Methylation
- Sequence Analysis, DNA