P-SAMS: a web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design.
basic_science · Level V
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- Record sourced from PubMed, PMID 26382195.
- Also identified by DOI 10.1093/bioinformatics/btv534 and PMC identifier 4681993.
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Abstract
The Plant Small RNA Maker Site (P-SAMS) is a web tool for the simple and automated design of artificial miRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) for efficient and specific targeted gene silencing in plants. P-SAMS includes two applications, P-SAMS amiRNA Designer and P-SAMS syn-tasiRNA Designer. The navigation through both applications is wizard-assisted, and the job runtime is relatively short. Both applications output the sequence of designed small RNA(s), and the sequence of the two oligonucleotides required for cloning into 'B/c' compatible vectors. The P-SAMS website is available at http://p-sams.carringtonlab.org. acarbonell@ibmcp.upv.es or nfahlgren@danforthcenter.org.
Medical subject headings
- Internet
- MicroRNAs
- Plants
- RNA, Plant
- RNA, Small Interfering
- Software