Real-time fluorescence imaging with 20 nm axial resolution.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26392382.
- Also identified by DOI 10.1038/ncomms9307 and PMC identifier 4595625.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Measuring the nanoscale organization of protein structures near the plasma membrane of live cells is challenging, especially when the structure is dynamic. Here we present the development of a two-wavelength total internal reflection fluorescence method capable of real-time imaging of cellular structure height with nanometre resolution. The method employs a protein of interest tagged with two different fluorophores and imaged to obtain the ratio of emission in the two channels. We use this approach to visualize the nanoscale organization of microtubules and endocytosis of the epidermal growth factor receptor.
Medical subject headings
- Cell Membrane
- Optical Imaging
- Proteins