Magnetic Resonance Detection of CD34+ Cells from Umbilical Cord Blood Using a 19F Label.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26394043.
- Also identified by DOI 10.1371/journal.pone.0138572 and PMC identifier 4578896.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Impaired homing and delayed recovery upon hematopoietic stem cell transplantation (HSCT) with hematopoietic stem cells (HSC) derived from umbilical cord blood (UCB) is a major problem. Tracking transplanted cells in vivo will be helpful to detect impaired homing at an early stage and allows early interventions to improve engraftment and outcome after transplantation. In this study, we show sufficient intracellular labeling of UCB-derived CD34+ cells, with 19F-containing PLGA nanoparticles which were detectable with both flow cytometry and magnetic resonance spectroscopy (MRS). In addition, labeled CD34+ cells maintain their capacity to proliferate and differentiate, which is pivotal for successful engraftment after transplantation in vivo. These results set the stage for in vivo tracking experiments, through which the homing efficiency of transplanted cells can be studied.
Medical subject headings
- Antigens, CD34
- Cell Tracking
- Fetal Blood
- Hematopoietic Stem Cells
- Magnetic Resonance Spectroscopy