Subangstrom single-molecule measurements of motor proteins using a nanopore.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26414351.
- Also identified by DOI 10.1038/nbt.3357 and PMC identifier 4915380.
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Abstract
Techniques for measuring the motion of single motor proteins, such as FRET and optical tweezers, are limited to a resolution of ∼300 pm. We use ion current modulation through the protein nanopore MspA to observe translocation of helicase Hel308 on DNA with up to ∼40 pm sensitivity. This approach should be applicable to any protein that translocates on DNA or RNA, including helicases, polymerases, recombinases and DNA repair enzymes.
Medical subject headings
- DNA
- DNA Helicases
- Micromanipulation
- Molecular Motor Proteins
- Nanopores