SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26423723.
- Also identified by DOI 10.1038/ncomms9497 and PMC identifier 4600740.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR-Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.
Medical subject headings
- DNA
- Fluorescent Dyes