Design of a New Type of Compact Chemical Heater for Isothermal Nucleic Acid Amplification.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26430883.
- Also identified by DOI 10.1371/journal.pone.0139449 and PMC identifier 4591995.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Previous chemical heater designs for isothermal nucleic acid amplification have been based on solid-liquid phase transition, but using this approach, developers have identified design challenges en route to developing a low-cost, disposable device. Here, we demonstrate the feasibility of a new heater configuration suitable for isothermal amplification in which one reactant of an exothermic reaction is a liquid-gas phase-change material, thereby eliminating the need for a separate phase-change compartment. This design offers potentially enhanced performance and energy density compared to other chemical and electric heaters.
Medical subject headings
- Nucleic Acid Amplification Techniques