Performance of Four Transport and Storage Systems for Molecular Detection of Multidrug-Resistant Tuberculosis.
Where this comes from
- Record sourced from PubMed, PMID 26431352.
- Also identified by DOI 10.1371/journal.pone.0139382 and PMC identifier 4591989.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
BACKGROUND: Detection of drug-resistant tuberculosis is essential for the control of the disease but it is often hampered by the limitation of transport and storage of samples from remote locations to the reference laboratory. We performed a retrospective field study to evaluate the performance of four supports enabling the transport and storage of samples to be used for molecular detection of drug resistance using the GenoType MTBDRplus. METHODS: Two hundred Mycobacterium tuberculosis strains were selected and spotted on slides, FTA cards, GenoCards, and in ethanol. GenoType MTBDRplus was subsequently performed with the DNA extracted from these supports. Sensitivity and specificity were calculated and compared to the results obtained by drug susceptibility testing. RESULTS: For all supports, the overall sensitivity and specificity for detection of resistance to RIF was between 95% and 100%, and for INH between 95% and 98%. CONCLUSION: The four transport and storage supports showed a good sensitivity and specificity for the detection of resistance to RIF and INH in M. tuberculosis strains using the GenoType MTBDRplus. These supports can be maintained at room temperature and could represent an important alternative cost-effective method useful for rapid molecular detection of drug-resistant TB in low-resource settings.
Medical subject headings
- Bacteriological Techniques
- DNA, Bacterial
- Drug Resistance, Multiple, Bacterial
- Molecular Diagnostic Techniques
- Mycobacterium tuberculosis
- Preservation, Biological
- Specimen Handling
- Sputum
- Transportation
- Tuberculosis, Multidrug-Resistant