A Putative PP2C-Encoding Gene Negatively Regulates ABA Signaling in Populus euphratica.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26431530.
- Also identified by DOI 10.1371/journal.pone.0139466 and PMC identifier 4592019.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
A PP2C homolog gene was cloned from the drought-treated cDNA library of Populus euphratica. Multiple sequence alignment analysis suggested that the gene is a potential ortholog of HAB1. The expression of this HAB1 ortholog (PeHAB1) was markedly induced by drought and moderately induced by ABA. To characterize its function in ABA signaling, we generated transgenic Arabidopsis thaliana plants overexpressing this gene. Transgenic lines exhibited reduced responses to exogenous ABA and reduced tolerance to drought compared to wide-type lines. Yeast two-hybrid analyses indicated that PeHAB1 could interact with the ABA receptor PYL4 in an ABA-independent manner. Taken together; these results indicated that PeHAB1 is a new negative regulator of ABA responses in poplar.
Medical subject headings
- Arabidopsis Proteins
- Gene Expression Regulation, Plant
- Populus