A strategy for dissecting the architectures of native macromolecular assemblies.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26436480.
- Also identified by DOI 10.1038/nmeth.3617 and PMC identifier 4803312.
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Abstract
It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures.
Medical subject headings
- Mass Spectrometry
- Models, Molecular
- Multiprotein Complexes
- Protein Conformation