SIMToolbox: a MATLAB toolbox for structured illumination fluorescence microscopy.
basic_science · Level V
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- Record sourced from PubMed, PMID 26446133.
- Also identified by DOI 10.1093/bioinformatics/btv576.
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Abstract
SIMToolbox is an open-source, modular set of functions for MATLAB equipped with a user-friendly graphical interface and designed for processing two-dimensional and three-dimensional data acquired by structured illumination microscopy (SIM). Both optical sectioning and super-resolution applications are supported. The software is also capable of maximum a posteriori probability image estimation (MAP-SIM), an alternative method for reconstruction of structured illumination images. MAP-SIM can potentially reduce reconstruction artifacts, which commonly occur due to refractive index mismatch within the sample and to imperfections in the illumination. SIMToolbox, example data and the online documentation are freely accessible at http://mmtg.fel.cvut.cz/SIMToolbox. ghagen@uccs.edu Supplementary data are available at Bioinformatics online.
Medical subject headings
- Actin Cytoskeleton
- Fluorescence
- Lighting
- Microscopy, Fluorescence
- Software