Metabolic Engineering of Klebsiella pneumoniae for the Production of 2-Butanone from Glucose.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26465746.
- Also identified by DOI 10.1371/journal.pone.0140508 and PMC identifier 4605612.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
2-Butanone is an important commodity chemical of wide application in different areas. In this study, Klebsiella pneumoniae was engineered to directly produce 2-butanone from glucose by extending its native 2, 3-butanediol synthesis pathway. To identify the potential enzyme for the efficient conversion of 2, 3-butanediol to 2-butanone, we screened different glycerol dehydratases and diol dehydratases. By introducing the diol dehydratase from Lactobacillus brevis and deleting the ldhA gene encoding lactate dehydrogenase, the engineered K. pneumoniae was able to accumulate 246 mg/L of 2-butanone in shake flask. With further optimization of culture condition, the titer of 2-butanone was increased to 450 mg/L. This study lays the basis for developing an efficient biological process for 2-butanone production.
Medical subject headings
- Butanones
- Glucose
- Klebsiella pneumoniae
- Metabolic Engineering