DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26479191.
- Also identified by DOI 10.1038/nbt.3389.
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Abstract
Editing plant genomes without introducing foreign DNA into cells may alleviate regulatory concerns related to genetically modified plants. We transfected preassembled complexes of purified Cas9 protein and guide RNA into plant protoplasts of Arabidopsis thaliana, tobacco, lettuce and rice and achieved targeted mutagenesis in regenerated plants at frequencies of up to 46%. The targeted sites contained germline-transmissible small insertions or deletions that are indistinguishable from naturally occurring genetic variation.
Medical subject headings
- CRISPR-Cas Systems
- Genetic Engineering
- Genome, Plant
- Plants, Genetically Modified
- Ribonucleoproteins