caRpools: an R package for exploratory data analysis and documentation of pooled CRISPR/Cas9 screens.

Winter, Jan; Breinig, Marco; Heigwer, Florian; Brügemann, Dirk; Leible, Svenja; Pelz, Oliver; Zhan, Tianzuo; Boutros, Michael · Bioinformatics · 2016

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Abstract

Genetic screens by CRISPR/Cas9-mediated genome engineering have become a powerful tool for functional genomics. However, there is currently a lack of end-to-end software pipelines to analyze CRISPR/Cas9 screens based on next generation sequencing. The CRISPR-AnalyzeR for pooled screens (caRpools) is an R package for exploratory data analysis that provides a complete workflow to analyze CRISPR/Cas9 screens. To further support the analysis of large-scale screens, caRpools integrates screening documentation and generation of standardized analysis reports. caRpools, manuals and an open virtual appliance are available at http://github.com/boutroslab/caRpools.

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