Measurement of Ensemble TRPV1 Ion Channel Currents Using Droplet Bilayers.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26513481.
- Also identified by DOI 10.1371/journal.pone.0141366 and PMC identifier 4626236.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Electrophysiological characterization of ion channels is useful for elucidation of channel function as well as quantitative assessment of pharmaceutical effects on ion channel conductance. We used droplet bilayers to measure ensemble ion channel currents from membrane preparations made from TRPV1-expressing HEK cells. Conductance measurements showed rectification, activation by acid and capsaicin, and inhibition by capsazepine, SB 452533, and JNJ 17293212. We also quantitatively measured concentration-dependent inhibition of channel conductance through determination of capsazepine IC50 in agreement with previously published studies using patch clamp. These results, combined with the reduced apparatus and material requirements of droplet bilayers, indicate that this platform could be used for study of other physiologically relevant ion channels.
Medical subject headings
- Action Potentials
- Lipid Bilayers
- TRPV Cation Channels