Thermal proteome profiling monitors ligand interactions with cellular membrane proteins.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26524241.
- Also identified by DOI 10.1038/nmeth.3652.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We extended thermal proteome profiling to detect transmembrane protein-small molecule interactions in cultured human cells. When we assessed the effects of detergents on ATP-binding profiles, we observed shifts in denaturation temperature for ATP-binding transmembrane proteins. We also observed cellular thermal shifts in pervanadate-induced T cell-receptor signaling, delineating the membrane target CD45 and components of the downstream pathway, and with drugs affecting the transmembrane transporters ATP1A1 and MDR1.
Medical subject headings
- Membrane Proteins
- Proteome
- Proteomics
- Tandem Mass Spectrometry