Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26567472.
- Also identified by DOI 10.1002/adma.201503939 and PMC identifier 4708057.
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Abstract
Pore-gradient microgel arrays enable thousands of parallel high-resolution single-cell protein electrophoresis separations for targets accross a wide molecular mass (25-289 kDa), yet within 1 mm separation distances. Dual crosslinked hydrogels facilitate gel-pore expansion after electrophoresis for efficient and uniform immunoprobing. The photopatterned, light-activated, and acid-expandable hydrogel underpins single-cell protein analysis, here for oncoprotein-related signaling in human breast biopsy.
Medical subject headings
- Blotting, Western
- Hydrogels
- Single-Cell Analysis