Sunflower-type nanogels carrying a quantum dot nanoprobe for both superior gene delivery efficacy and tracing of human mesenchymal stem cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26576046.
- Also identified by DOI 10.1016/j.biomaterials.2015.11.002.
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Abstract
Sunflower-type nanogels carrying the QD 655 nanoprobe can be used for both gene transfection and bioimaging of hMSCs. The entry of sunflower-type nanogels into hMSCs can be possibly controlled by changing the formation of QDs. The physico-chemical properties of sunflower-type nanogels internalized by hMSCs were confirmed by AFM, SEM, TEM, gel retardation, and ζ-potential analyses. The bioimaging capacity was confirmed by confocal laser microscopy, Kodak imaging, and Xenogen imaging. Specifically, we investigated the cytotoxicity of sunflower-type nanogels via SNP analysis. Internalization of sunflower-type nanogels does not cause malfunction of hMSCs.
Medical subject headings
- Cell Tracking
- Mesenchymal Stem Cell Transplantation
- Polyethylene Glycols
- Polyethyleneimine
- Quantum Dots
- Transfection