Electron Microscopy of Living Cells During in Situ Fluorescence Microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26580231.
- Also identified by DOI 10.1021/acsnano.5b03970 and PMC identifier 4729641.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We present an approach toward dynamic nanoimaging: live fluorescence of cells encapsulated in a bionanoreactor is complemented with in situ scanning electron microscopy (SEM) on an integrated microscope. This allows us to take SEM snapshots on-demand, that is, at a specific location in time, at a desired region of interest, guided by the dynamic fluorescence imaging. We show that this approach enables direct visualization, with EM resolution, of the distribution of bioconjugated quantum dots on cellular extensions during uptake and internalization.
Medical subject headings
- Cells, Immobilized
- Fibroblasts
- Microscopy, Electron, Scanning
- Optical Imaging