NeuroGPS-Tree: automatic reconstruction of large-scale neuronal populations with dense neurites.

Quan, Tingwei; Zhou, Hang; Li, Jing; Li, Shiwei; Li, Anan; Li, Yuxin; Lv, Xiaohua; Luo, Qingming et al. · Nat Methods · 2016

basic_science · Level V

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Abstract

The reconstruction of neuronal populations, a key step in understanding neural circuits, remains a challenge in the presence of densely packed neurites. Here we achieved automatic reconstruction of neuronal populations by partially mimicking human strategies to separate individual neurons. For populations not resolvable by other methods, we obtained recall and precision rates of approximately 80%. We also demonstrate the reconstruction of 960 neurons within 3 h.

Medical subject headings