Large area magnetic micropallet arrays for cell colony sorting.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26606460.
- Also identified by DOI 10.1039/c5lc01131k and PMC identifier 6201277.
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Abstract
A new micropallet array platform for adherent cell colony sorting has been developed. The platform consisted of thousands of square plastic pallets, 270 μm by 270 μm on each side, large enough to hold a single colony of cells. Each pallet included a magnetic core, allowing them to be collected with a magnet after being released using a microscope mounted laser system. The micropallets were patterned from 1002F epoxy resist and were fabricated on translucent, gold coated microscope slides. The gold layer was used as seed for electroplating the ferromagnetic cores within every individual pallet. The gold layer also facilitated the release of each micropallet during laser release. This array allows for individual observation, sorting and collection of isolated cell colonies for biological cell colony research. In addition to consistent release and recovery of individual colonies, we demonstrated stable biocompatibility and minimal loss in imaging quality compared to previously developed micropallet arrays.
Medical subject headings
- Cell Culture Techniques
- Cell Separation
- Magnetic Phenomena
- Tissue Array Analysis