Electrophoretic Deformation of Individual Transfer RNA Molecules Reveals Their Identity.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26609994.
- Also identified by DOI 10.1021/acs.nanolett.5b03331 and PMC identifier 4890568.
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Abstract
It has been hypothesized that the ribosome gains additional fidelity during protein translation by probing structural differences in tRNA species. We measure the translocation kinetics of different tRNA species through ∼3 nm diameter synthetic nanopores. Each tRNA species varies in the time scale with which it is deformed from equilibrium, as in the translocation step of protein translation. Using machine-learning algorithms, we can differentiate among five tRNA species, analyze the ratios of tRNA binary mixtures, and distinguish tRNA isoacceptors.
Medical subject headings
- Nanopores
- Protein Biosynthesis
- RNA, Transfer