Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26619013.
- Also identified by DOI 10.1038/nmeth.3667 and PMC identifier 4890631.
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Abstract
We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II (<3-Å diffraction) and Thermus thermophilus small ribosomal subunit bound to the antibiotic paromomycin at ambient temperature (3.4-Å structure).
Medical subject headings
- Crystallography
- Photosystem II Protein Complex
- Ribosomes