Site-Specific Polymer Conjugation Stabilizes Therapeutic Enzymes in the Gastrointestinal Tract.

Schulz, Jessica D; Patt, Melanie; Basler, Sophie; Kries, Hajo; Hilvert, Donald; Gauthier, Marc A; Leroux, Jean-Christophe · Adv Mater · 2016

basic_science · Level V

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Abstract

The site-specific conjugation of polymers to multiple engineered cysteine residues of a prolyl endopeptidase leads to its stabilization in the gastrointestinal tract of rats, without compromising the activity relative to the native enzyme. The importance of polymer attachment sites is investigated, as well as the significance of polymer structure.

Medical subject headings