B Cells Loaded with Synthetic Particulate Antigens: A Versatile Platform To Generate Antigen-Specific Helper T Cells for Cell Therapy.

Sicard, Antoine; Koenig, Alice; Graff-Dubois, Stéphanie; Dussurgey, Sébastien; Rouers, Angéline; Dubois, Valérie; Blanc, Pascal; Chartoire, Dimitri et al. · Nano Lett · 2016

basic_science · Level V

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Abstract

Adoptive cell therapy represents a promising approach for several chronic diseases. This study describes an innovative strategy for biofunctionalization of nanoparticles, allowing the generation of synthetic particulate antigens (SPAg). SPAg activate polyclonal B cells and vectorize noncognate proteins into their endosomes, generating highly efficient stimulators for ex vivo expansion of antigen-specific CD4+ T cells. This method also allows harnessing the ability of B cells to polarize CD4+ T cells into effectors or regulators.

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