Inverted light-sheet microscope for imaging mouse pre-implantation development.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26657559.
- Also identified by DOI 10.1038/nmeth.3690.
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Abstract
Despite its importance for understanding human infertility and congenital diseases, early mammalian development has remained inaccessible to in toto imaging. We developed an inverted light-sheet microscope that enabled us to image mouse embryos from zygote to blastocyst, computationally track all cells and reconstruct a complete lineage tree of mouse pre-implantation development. We used this unique data set to show that the first cell fate specification occurs at the 16-cell stage.
Medical subject headings
- Blastocyst
- Microscopy