KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light.
basic_science · Level V
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- Record sourced from PubMed, PMID 26679300.
- Also identified by DOI 10.1371/journal.pone.0145287 and PMC identifier PMC3804158.
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Abstract
Genetically encoded photosensitizers, proteins that produce reactive oxygen species when illuminated with visible light, are increasingly used as optogenetic tools. Their applications range from ablation of specific cell populations to precise optical inactivation of cellular proteins. Here, we report an orange mutant of red fluorescent protein KillerRed that becomes toxic when illuminated with blue or green light. This new protein, KillerOrange, carries a tryptophan-based chromophore that is novel for photosensitizers. We show that KillerOrange can be used simultaneously and independently from KillerRed in both bacterial and mammalian cells offering chromatic orthogonality for light-activated toxicity.