Genome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26748756.
- Also identified by DOI 10.1016/j.stem.2015.12.002 and PMC identifier 4709030.
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Abstract
Human pluripotent stem cells (hPSCs) with knockout or mutant alleles can be generated using custom-engineered nucleases. Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 nucleases are the most commonly employed technologies for editing hPSC genomes. In this Protocol Review, we provide a brief overview of custom-engineered nucleases in the context of gene editing in hPSCs with a focus on the application of TALENs and CRISPR/Cas9. We will highlight the advantages and disadvantages of each method and discuss theoretical and technical considerations for experimental design.
Medical subject headings
- CRISPR-Cas Systems
- Genetic Techniques
- Genome, Human
- Pluripotent Stem Cells