Predictable tuning of protein expression in bacteria.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26752768.
- Also identified by DOI 10.1038/nmeth.3727.
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Abstract
We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOPEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOPEC is a free tool that makes it possible to modulate the expression level of any Escherichia coli gene by changing only a few bases. Measured protein levels for 91% of our designed sequences were within twofold of the desired target level.
Medical subject headings
- Escherichia coli
- Escherichia coli Proteins
- Peptide Chain Initiation, Translational
- Protein Engineering
- RNA, Bacterial
- Software