Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity.
basic_science · Level V
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- Record sourced from PubMed, PMID 26752769.
- Also identified by DOI 10.1038/nmeth.3728 and PMC identifier 4770512.
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Abstract
We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing that allows for the discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method revealed previously unrecognized associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.
Medical subject headings
- Embryonic Stem Cells
- Epigenesis, Genetic
- High-Throughput Nucleotide Sequencing
- Regulatory Elements, Transcriptional
- Transcription Factors