Characterizing transcriptional heterogeneity through pathway and gene set overdispersion analysis.
basic_science · Level V
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- Record sourced from PubMed, PMID 26780092.
- Also identified by DOI 10.1038/nmeth.3734 and PMC identifier 4772672.
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Abstract
The transcriptional state of a cell reflects a variety of biological factors, from cell-type-specific features to transient processes such as the cell cycle, all of which may be of interest. However, identifying such aspects from noisy single-cell RNA-seq data remains challenging. We developed pathway and gene set overdispersion analysis (PAGODA) to resolve multiple, potentially overlapping aspects of transcriptional heterogeneity by testing gene sets for coordinated variability among measured cells.
Medical subject headings
- Gene Expression Profiling
- Proteome
- Sequence Analysis, RNA
- Signal Transduction
- Transcription, Genetic
- Transcriptome