An informatic framework for decoding protein complexes by top-down mass spectrometry.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26780093.
- Also identified by DOI 10.1038/nmeth.3731 and PMC identifier 4767540.
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Abstract
Efforts to map the human protein interactome have resulted in information about thousands of multi-protein assemblies housed in public repositories, but the molecular characterization and stoichiometry of their protein subunits remains largely unknown. Here, we report a computational search strategy that supports hierarchical top-down analysis for precise identification and scoring of multi-proteoform complexes by native mass spectrometry.
Medical subject headings
- Data Mining
- Databases, Protein
- Mass Spectrometry
- Protein Interaction Mapping
- Proteome
- Sequence Analysis, Protein