Identification of the Sfp-Type PPTase EppA from the Lichenized Fungus Evernia prunastri.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26784935.
- Also identified by DOI 10.1371/journal.pone.0145624 and PMC identifier 4718654.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
In the last decades, natural products from lichens have gained more interest for pharmaceutical application due to the broad range of their biological activity. However, isolation of the compounds of interest directly from the lichen is neither feasible nor sustainable due to slow growth of many lichens. In order to develop a pipeline for heterologous expression of lichen biosynthesis gene clusters and thus the sustainable production of their bioactive compounds we have identified and characterized the phosphopantheteinyl transferase (PPTase) EppA from the lichen Evernia prunastri. The Sfp-type PPTase EppA was functionally characterized through heterologous expression in E. coli using the production of the blue pigment indigoidine as readout and by complementation of a lys5 deletion in S. cerevisiae.
Medical subject headings
- Bacterial Proteins
- Lichens
- Transferases (Other Substituted Phosphate Groups)