SMYD1, an SRF-Interacting Partner, Is Involved in Angiogenesis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26799706.
- Also identified by DOI 10.1371/journal.pone.0146468 and PMC identifier 4723226.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Previous studies have demonstrated that Smyd1 plays a critical role in cardiomyocyte differentiation, cardiac morphogenesis and myofibril organization. In this study, we uncovered a novel function of Smyd1 in the regulation of endothelial cells (ECs). Our data showed that Smyd1 is expressed in vascular endothelial cells, and knockdown of SMYD1 in endothelial cells impairs EC migration and tube formation. Furthermore, Co-IP and GST pull-down assays demonstrated that SMYD1 is associated with the Serum Response Factor (SRF). EMSA assays further showed that SMYD1 forms a complex with SRF and enhances SRF DNA binding activity. Our studies indicate that SMYD1 serves as an SRF-interacting protein, enhances SRF DNA binding activity, and is required for EC migration and tube formation to regulate angiogenesis.
Medical subject headings
- DNA-Binding Proteins
- Human Umbilical Vein Endothelial Cells
- Morphogenesis
- Muscle Development
- Muscle Proteins
- Neovascularization, Physiologic
- Serum Response Factor
- Transcription Factors