Highly multiplexed simultaneous detection of RNAs and proteins in single cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26808670.
- Also identified by DOI 10.1038/nmeth.3742 and PMC identifier 4767631.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
To enable the detection of expression signatures specific to individual cells, we developed PLAYR (proximity ligation assay for RNA), a method for highly multiplexed transcript quantification by flow and mass cytometry that is compatible with standard antibody staining. When used with mass cytometry, PLAYR allowed for the simultaneous quantification of more than 40 different mRNAs and proteins. In primary cells, we quantified multiple transcripts, with the identity and functional state of each analyzed cell defined on the basis of the expression of a separate set of transcripts or proteins. By expanding high-throughput deep phenotyping of cells beyond protein epitopes to include RNA expression, PLAYR opens a new avenue for the characterization of cellular metabolism.
Medical subject headings
- Flow Cytometry
- High-Throughput Nucleotide Sequencing
- High-Throughput Screening Assays
- Protein Array Analysis
- Proteins
- RNA