T Cell Receptor Mediated Calcium Entry Requires Alternatively Spliced Cav1.1 Channels.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26815481.
- Also identified by DOI 10.1371/journal.pone.0147379 and PMC identifier 4729531.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The process of calcium entry in T cells is a multichannel and multi-step process. We have studied the requirement for L-type calcium channels (Cav1.1) α1S subunits during calcium entry after TCR stimulation. High expression levels of Cav1.1 channels were detected in activated T cells. Sequencing and cloning of Cav1.1 channel cDNA from T cells revealed that a single splice variant is expressed. This variant lacks exon 29, which encodes the linker region adjacent to the voltage sensor, but contains five new N-terminal exons that substitute for exons 1 and 2, which are found in the Cav1.1 muscle counterpart. Overexpression studies using cloned T cell Cav1.1 in 293HEK cells (that lack TCR) suggest that the gating of these channels was altered. Knockdown of Cav1.1 channels in T cells abrogated calcium entry after TCR stimulation, suggesting that Cav1.1 channels are controlled by TCR signaling.
Medical subject headings
- CD4-Positive T-Lymphocytes
- Calcium
- Calcium Channels, L-Type
- Receptors, Antigen, T-Cell