Volta phase plate cryo-EM of the small protein complex Prx3.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26817416.
- Also identified by DOI 10.1038/ncomms10534 and PMC identifier 4738354.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Cryo-EM of large, macromolecular assemblies has seen a significant increase in the numbers of high-resolution structures since the arrival of direct electron detectors. However, sub-nanometre resolution cryo-EM structures are rare compared with crystal structure depositions, particularly for relatively small particles (<400 kDa). Here we demonstrate the benefits of Volta phase plates for single-particle analysis by time-efficient cryo-EM structure determination of 257 kDa human peroxiredoxin-3 dodecamers at 4.4 Å resolution. The Volta phase plate improves the applicability of cryo-EM for small molecules and accelerates structure determination.
Medical subject headings
- Cryoelectron Microscopy
- Multiprotein Complexes
- Peroxiredoxin III