NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26839278.
- Also identified by DOI 10.1038/ncomms10597 and PMC identifier 4742958.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
In vertebrate cells, chromosomes oscillate to align precisely during metaphase. NuSAP, a microtubule-associated protein, plays a critical role in stabilizing spindle microtubules. In this study, we utilize 3D time-lapse live-cell imaging to monitor the role of NuSAP in chromosome oscillation and identify NuSAP as a novel regulator of the chromokinesin, Kid. Depletion of NuSAP significantly suppresses the amplitude and velocity of chromosome oscillation. We analyse the effects of NuSAP and Kid depletion in monopolar and bipolar cells with or without kinetochore microtubule depletion. Twelve postulated conditions are deciphered to reveal the contribution of NuSAP to the polar force generated at kinetochore microtubules and to the regulation of the polar ejection force generated by Kid, thus revealing a pivotal role of NuSAP in chromosome oscillation.
Medical subject headings
- Chromosomes
- DNA-Binding Proteins
- Kinesins
- Metaphase
- Microtubule-Associated Proteins
- Microtubules
- Movement