Development of an Indirect ELISA for Serological Diagnosis of Bovine herpesvirus 5.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26866923.
- Also identified by DOI 10.1371/journal.pone.0149134 and PMC identifier 4750905.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Bovine herpesviruses 1 and 5 (BoHV-1 and BoHV-5) are economically important pathogens, associated with a variety of clinical syndromes, including respiratory and genital disease, reproductive failure and meningoencephalitis. The standard serological assay to diagnose BoHV-1 and BoHV-5 infections is the virus neutralization test (VNT), a time consuming procedure that requires manipulation of infectious virus. In the present study a highly sensitive and specific single dilution indirect ELISA was developed using recombinant glycoprotein D from BoHV-5 as antigen (rgD5ELISA). Bovine serum samples (n = 450) were screened by VNT against BoHV-5a and by rgD5ELISA. Compared with the VNT, the rgD5ELISA demonstrated accuracy of 99.8%, with 100% sensitivity, 96.7% specificity and coefficient of agreement between the tests of 0.954. The rgD5ELISA described here shows excellent agreement with the VNT and is shown to be a simple, convenient, specific and highly sensitive virus-free assay for detection of serum antibodies to BoHV-5.
Medical subject headings
- Cattle Diseases
- Encephalitis, Viral
- Enzyme-Linked Immunosorbent Assay
- Herpesviridae Infections
- Meningoencephalitis
- Viral Envelope Proteins